|Surface Activation of Inorganic and Organic Biomaterials |
Laboratory for Surface Modification
|Jefferey Schwartz, Princeton University|
12:00 Noon, Chem. 260
A modular approach has been developed to control cell-surface interactions, which is based on analysis of substrate surface chemistry. Methods were devised to prepare self-assembled monolayers of phosphonates (SAMPs) on a variety of metals, silicon, and organic polymers. The monolayers are thin (about 2 nm) and are easily applied throughout porous structures. They adhere strongly to metallic surfaces and are stable to hydrolysis. The chemistry of these monolayers allows for bonding of cell-adherent peptides to the substrate surfaces. In other words, the SAMPs serve as thin and stable interfaces between substrates and cells.
Analysis of treated surfaces can be accomplished quantitatively, and surface yields for peptide attachment are high, either on metals or polymers. Cell growth and spreading on treated surfaces is greater than on controls, and an in vivo test showed that bone growth on a porous implant was far greater than on one treated with hydroxyapatite, which is a commercially accepted surface treatment.